Gluten Testing
ELISA-based gluten detection down to trace levels, so your "gluten-free" claim is backed by verified data against the 20 ppm threshold used globally and by FSSAI.
What is gluten testing?
Gluten testing measures the gluten content of a food product, ingredient or equipment surface and checks it against the 20 ppm threshold recognised by Codex Alimentarius and FSSAI. For people with coeliac disease or gluten sensitivity, trace contamination isn't a quality issue. It's a health event. A "gluten-free" label carries real weight, and cross-contact from shared production lines, contaminated raw materials or mislabelled ingredients can undermine that claim without anyone in the plant noticing.
Equinox Labs tests for gluten content using ELISA-based methods sensitive enough to detect trace contamination, benchmarked against the 20 ppm gluten-free threshold recognised internationally and by FSSAI, trusted by 95,000+ clients across industries. We test raw ingredients, finished products and even swab samples from shared processing equipment. It's often tested alongside our broader food safety testing and naturally occurring toxins services.
Why does an unverified gluten-free claim put you at risk?
Four consequences that follow an untested batch.
- Consumer health risk. Coeliac disease and gluten sensitivity mean trace contamination can trigger a real reaction, not just a quality complaint.
- Regulatory exposure. An unsubstantiated claim breaches FSSAI, BIS and international food labelling regulations.
- Shelf-life uncertainty. Accurate gluten data underpins reliable shelf-life predictions for gluten-free formulations.
- Blocked market access. Export and private-label buyers increasingly require documented gluten testing before listing a product.
What does gluten testing check across your supply chain?
From raw material intake to shared-line cross-contact checks.
Raw Material Verification
Confirms incoming flours, starches and additives are genuinely gluten-free before use.
Finished Product Testing
Batch release testing to validate "gluten-free" label claims before dispatch.
Cross-Contact Swab Testing
Equipment and surface swabs on shared production lines to catch cross-contamination risk.
Multi-Method Confirmatory Testing
HPLC and LC-MS/MS available alongside ELISA for complex matrices and trace-level confirmation.
Export Threshold Testing
Benchmarking against FDA, EU and other destination-market gluten-free limits for export-bound goods.
Retail & Private Label Verification
Independent third-party testing retailers and private-label buyers can rely on before listing.
What does the test screen for?
Gluten protein fractions from wheat, barley and rye sources.
Sources
Quantification
Sample Types
Who needs gluten testing?
From specialty bakeries to shared-facility manufacturers and export brands.
Gluten-Free Food Brands
Validate every batch before it carries a "gluten-free" claim on shelf.
Shared-Line Manufacturers
Facilities producing both gluten-containing and gluten-free SKUs on the same equipment.
Ingredient Suppliers
Third-party verification of gluten-free starches, flours and additives sold to manufacturers.
Bakeries & Snack Producers
High cross-contamination risk from flour dust and shared ovens, mixers and utensils.
Retailers & Private Label Brands
Independent verification before a supplier's product carries your retail label.
Export-Focused Brands
Testing against FDA, EU and other international thresholds ahead of shipment.
Sample to certified report, in four steps
The same process across every gluten testing engagement.
Inquire
Tell us whether you need ingredient, finished product or swab testing.
Sample Collection
Send in samples or have our team collect from your facility.
ELISA Testing
Samples are analysed for gluten content at our NABL-accredited laboratories.
Results & Support
We email your certified report and advise on any corrective action needed.
Access the Equinox PortalTrace-level accuracy for a claim that can't be wrong
Accredited ELISA methodology and a team that understands cross-contact risk.
NABL Accredited
ISO/IEC 17025 accredited testing with detection sensitivity down to trace levels.
Codex & FSSAI Aligned
Benchmarked against the internationally recognised 20 ppm gluten-free threshold.
Pan-India Reach
Sample logistics and testing coverage across 500+ cities, trusted by 95,000+ clients.
A Dedicated Account Manager
One point of contact for recurring batch and cross-contact monitoring.
Gluten testing FAQs
Gluten testing measures the gluten content of a food product, ingredient or equipment surface using laboratory methods such as ELISA, HPLC or LC-MS/MS, then checks the result against the 20 ppm gluten-free threshold recognised by Codex Alimentarius and FSSAI.
The internationally recognised threshold (Codex Alimentarius, also referenced by FSSAI) is 20 parts per million (ppm). Our ELISA method can detect gluten well below this level.
Gluten content is measured in parts per million (ppm) to verify whether a product meets the gluten-free or low-gluten threshold that applies to your market.
We primarily use ELISA (Enzyme-Linked Immunosorbent Assay) with R5 antibody-based kits, the industry-standard method recommended by Codex Alimentarius. For complex matrices or trace-level confirmatory testing, we also use HPLC and LC-MS/MS.
Yes. We swab shared production lines, mixers and utensils to detect residual gluten contamination between gluten-containing and gluten-free production runs.
We detect gluten proteins from wheat (gliadin), barley (hordein) and rye (secalin), the three grains that trigger a reaction in coeliac disease.
Yes. We benchmark against FSSAI and Codex limits as standard, and can test against FDA and other destination-market thresholds for export-bound gluten-free products.
Get your Gluten Testing enquiry answered
Tell us a little about your requirement and our team will get back to you within one working day.
- Response within one working day
- PAN-India service network
- Dedicated account manager
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